FE30

Fluorescence spectrometer

The FE30 is a high-sensitivity fluorescence spectrometer designed for fluorescence measurements in research, routine analysis and laboratory applications. It combines a sensitive optical system with fast spectral scanning and a wavelength range extending up to 900 nm.

With a water Raman signal-to-noise ratio of up to 20,000:1 RMS-BG and scan speeds up to 60,000 nm/min, the FE30 can measure low fluorescence signals while also supporting rapid acquisition of excitation, emission and 3D fluorescence spectra.

High-sensitivity fluorescence measurements

The FE30 combines an efficient optical and electronic system with a high-intensity 150 W xenon lamp and PMT detector. This configuration provides the sensitivity needed to detect relatively weak fluorescence signals and samples at low concentrations.

Under the specified water Raman measurement conditions, the FE30 achieves a signal-to-noise ratio of 1,000:1 RMS-PK and 20,000:1 RMS-BG.

Excitation and emission bandwidths can be selected from 1, 2.5, 5, 10 and 20 nm, allowing measurement conditions to be adjusted according to the required sensitivity and spectral resolution.

Fluorescence measurements up to 900 nm

Both the excitation and emission wavelength ranges extend from 200 to 900 nm. This enables the FE30 to measure fluorescence across the UV and visible regions and into longer wavelengths.

The extended range is particularly useful for fluorophores that absorb or emit at longer wavelengths and expands the range of samples that can be investigated with a single instrument.

Flexible fluorescence measurement modes

The FE30 supports several measurement modes through the Spectracle software, allowing the instrument to be used for both routine fluorescence measurements and more advanced sample characterization.

Excitation and emission scans

Excitation and emission spectra can be recorded to investigate the fluorescence characteristics of a sample. Synchronous wavelength scanning is also available.

Time scan

Kinetic measurements can be used to monitor fluorescence changes over time, including applications such as enzyme activity and other reactions.

3D fluorescence scan

3D scanning provides an overview of a sample’s fluorescence characteristics across excitation and emission wavelengths. The resulting excitation-emission maps can support sample characterization, fingerprint analysis and advanced modelling.

With scan speeds up to 60,000 nm/min, the FE30 can acquire the data required for 3D spectra within a few minutes.

Quantitation

Calibration curves can be generated for quantitative fluorescence analysis, including first- and second-order calibration. It is possible to then use these to determine unknown sample concentrations from their fluorescence response.

Fluorescence anisotropy

With the appropriate polarizer accessory, the FE30 can perform fluorescence polarization and anisotropy measurements for applications in areas such as biochemistry and biophysics.

Instrument validation

Built-in validation functions help laboratories check the performance of the FE30. The software supports automated water Raman scans for signal-to-noise assessment as well as excitation and emission wavelength accuracy checks.

This provides a convenient way to monitor instrument performance as part of routine laboratory workflows.

Sample handling and accessories

The FE30 has a large sample chamber that can accommodate different sample holders and accessories. This allows measurements to extend beyond standard liquid fluorescence samples to temperature-controlled liquids, powders and thin films.

Available options include:

• Standard cell holder for 10 mm fluorescence cuvettes
• Thermostatic water bath cell holder for temperature-controlled measurements
• Variable-angle solid sample holder for powders and thin films
• Micro powder sample holder for small quantities of powder
• Polarizer accessory for fluorescence anisotropy measurements
• Laser source holder for coupling an external excitation laser

The variable-angle solid sample holder supports continuous 360° rotation and measurement angles from 0 to 90°, while the thermostatic water bath holder supports measurements at approximately 0 to 100°C.

Applications

The FE30 can be used for fluorescence measurements across a range of research and analytical fields, including:

• Food analysis
• Molecular biology
• Chemistry
• Life sciences
• Environmental analysis
• Material science
• Semiconductor research