DB30

Double beam UV-Vis spectrophotometer

The DB30 is a double beam UV-Vis spectrophotometer designed for absorbance and transmittance measurements across the UV and visible spectrum. Covering a wavelength range from 190 to 1,100 nm, it combines selectable spectral bandwidths with fast scanning and flexible sample handling for routine analysis, research and quality-control applications.

The instrument can be operated directly through its integrated 7-inch touchscreen or controlled from a PC, allowing it to fit both standalone laboratory workflows and more advanced data-management environments.

Flexible UV-Vis measurements

The DB30 supports several measurement modes, allowing one instrument to handle different types of UV-Vis analysis.

Wavelength scanning

Absorbance, transmittance or detector signal can be measured across a selected wavelength range. Scan speed, wavelength increment and spectral bandwidth can be configured according to the measurement.

Time scan

For kinetic measurements, you can follow changes over time for measurement periods ranging from one minute to more than 27 hours.

Photometry

Measurements can be performed at individual wavelengths in either absorbance or transmittance mode. Up to ten individual wavelengths can be included in a measurement.

Quantitation

The built-in quantitation mode uses measurements from standard solutions to create calibration curves. Once the calibration has been established, the system calculates the concentrations of unknown samples from their absorbance values.

The touchscreen also includes application wizards for protein, nucleic acid and hexavalent chromium measurements.

Double beam optical system

The DB30 uses a Czerny-Turner double beam monochromator together with deuterium and tungsten-halogen light sources. The double beam configuration allows the sample and reference signals to be monitored during measurement, supporting stable measurements across the instrument’s wavelength range.

With selectable bandwidths from 0.5 to 4.0 nm, the system can be configured according to the spectral resolution required for a particular analysis. Scan speeds ranging from 10 to 6,000 nm/min provide additional flexibility between detailed spectral measurements and faster routine scanning.

UV measurements down to 190 nm

The DB30 covers wavelengths from 190 nm in the UV region through to 1,100 nm in the near-infrared region. Its photometric range extends from -3.4 to +3.4 Abs, enabling measurements across a broad absorbance range.

This makes the instrument suitable for samples and applications requiring measurements in the deeper UV region as well as conventional visible spectroscopy.

Touchscreen and PC operation

Every DB30 includes a touchscreen for standalone operation. Measurement setup, acquisition and data analysis can therefore be performed directly at the instrument without requiring a separate computer.

Data-processing functions available through the touchscreen include spectrum overlay, peak and trough analysis, smoothing, differentiation, arithmetic operations and area and rate calculations. Results can be stored internally or transferred using USB or SD card.

Sample handling and accessories

Depending on the application, it is possible to equip the DB30 for standard cuvettes, micro-volume samples, long-path measurements, temperature-controlled measurements, films, glass samples and continuous-flow analysis.

Available options include:

• Standard 10 mm cell holder
• Micro cell holder for 50 µl micro-volume cells
• Long-path cell holder for 10–100 mm optical path lengths
• Thermostatic cell holder
• Electronic temperature-controlled cell holder
• Six-position automatic cell holder
• Micro flow cell
• Auto sipper
• Glass filter holder
• Film holder
• SpectroLink

Applications

The DB30 can be used for UV-Vis measurements across a broad range of laboratory environments, including:

• Pharmaceutical analysis
• Food analysis
• Molecular biology
• Chemistry
• Life sciences
• Environmental analysis
• Petrochemical analysis